Abstract. Various current data, collected in the Strait of Otranto during the period 1994–2007, have been analysed with the aim of describing the characteristics of the tidal motions and their contribution to the total flow variance. The principal tidal constituents in the area were the semi-diurnal (M2) and the diurnal (K1), with the latter one predominant. The total flow was, in general, more energetic along the flanks than in the middle of the strait. Specifically, it was most energetic over the western shelf and in the upper layer along the eastern flank. In spite of the generally low velocities (a few cm s⁻¹) of the principal tidal constituents, the tidal variance has a pattern similar to that of the total flow variance, that is, it was large over the western shelf and low in the middle. The proportion of non-tidal (comprising the inertial and sub-inertial low-frequency bands) to tidal flow variances was quite variable in both time and space. The low-frequency motions dominated over the tidal and inertial ones in the eastern portion of the strait during the major part of the year, particularly in the upper and intermediate layers. In the deep, near-bottom layer the variance was evenly distributed between the low frequency, diurnal and semi-diurnal bands. An exception was observed near the western shelf break during the summer season when the contribution of the tidal signal to the total variance reached 77%. This high contribution was mainly due to the intensification of the diurnal signal at that location at both upper and bottom current records (velocities of about 10 cm s⁻¹). Local wind and sea level data were analysed and compared with the flow to find the possible origin of this diurnal intensification. Having excluded the sea-breeze impact on the intensification of the diurnal tidal signal, the most likely cause remains the generation of the topographically trapped internal waves and the diurnal resonance in the tidal response. These waves were sometimes generated by the barotropic tidal signal in the presence of summer stratification and the strong bottom slope. This phenomenon may stimulate diapycnal mixing during the stratified season and enhance ventilation of the near-bottom layers.