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Ocean Science An interactive open-access journal of the European Geosciences Union
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Volume 11, issue 6 | Copyright
Ocean Sci., 11, 937-946, 2015
https://doi.org/10.5194/os-11-937-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 27 Nov 2015

Research article | 27 Nov 2015

An improved method for the determination of dissolved nitric oxide (NO) in seawater samples

H. E. Lutterbeck and H. W. Bange H. E. Lutterbeck and H. W. Bange
  • Chemical Oceanography, Division of Marine Biogeochemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany

Abstract. Nitric oxide (NO) is a short-lived intermediate of the oceanic nitrogen cycle; however, due to its high reactivity, measurements of dissolved NO in seawater are rare. Here we present an improved method to determine NO concentrations in discrete seawater samples. The set-up of our system consisted of a chemiluminescence NO analyser connected to a stripping unit. The limit of detection for our method is 5 pmol NO in aqueous solution, which translates into 0.25 nmol L−1 when using a 20 mL seawater sample volume. Our method was applied to measure high-resolution depth profiles of dissolved NO during a cruise to the eastern tropical South Pacific Ocean. It is fast and comparably easy to handle; thus it opens the door for investigating the distribution of NO in the ocean, and it facilitates laboratory studies on NO pathways.

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