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OS | Articles | Volume 14, issue 5
Ocean Sci., 14, 1185-1206, 2018
https://doi.org/10.5194/os-14-1185-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Ocean Sci., 14, 1185-1206, 2018
https://doi.org/10.5194/os-14-1185-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 09 Oct 2018

Research article | 09 Oct 2018

Turbulence and hypoxia contribute to dense biological scattering layers in a Patagonian fjord system

Turbulence and hypoxia contribute to dense biological scattering layers
Iván Pérez-Santos1,2, Leonardo Castro2,3,4, Lauren Ross5, Edwin Niklitschek1, Nicolás Mayorga1, Luis Cubillos2,3, Mariano Gutierrez6, Eduardo Escalona2,3, Manuel Castillo2,7, Nicolás Alegría8, and Giovanni Daneri2,9 Iván Pérez-Santos et al.
  • 1Centro i-mar, Universidad de Los Lagos, Camino a Chinquihue km 6, Puerto Montt, Chile
  • 2Centro COPAS Sur-Austral, Universidad de Concepción, Campus Concepción, Víctor Lamas 1290, Casilla 160-C, código postal: 4070043, Concepción, Chile
  • 3Departamento de Oceanografía, Universidad de Concepción, Campus Concepción, Víctor Lamas 1290, Casilla 160-C, código postal: 4070043, Concepción, Chile
  • 4Centro de Investigaciones de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile
  • 5Department of Civil and Environmental Engineering, University of Maine, 5711 Boardman Hall, Orono, ME 04469-5711, USA
  • 6Universidad Nacional Federico Villareal, Facultad de Oceanografía, Pesquerías y Ciencias Alimentarias, Calle Francia 726, Miraflores, Lima, Peru
  • 7Centro de Observación Marino para Estudios de Riesgo en Ambientes Costeros, Facultad de Ciencias del Mar y de Recursos Naturales, Universidad de Valparaíso, Valparaiso, Chile
  • 8Instituto de Investigaciones Pesqueras, Talcahuano, Chile
  • 9Centro de Investigaciones en Ecosistemas de la Patagonia (CIEP), Coyhaique, Chile
Abstract
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The aggregation of plankton species along fjords can be linked to physical properties and processes such as stratification, turbulence and oxygen concentration. The goal of this study is to determine how water column properties and turbulent mixing affect the horizontal and vertical distributions of macrozooplankton along the only northern Patagonian fjord known to date, where hypoxic conditions occur in the water column. Acoustic Doppler current profiler moorings, scientific echo-sounder transects and in situ plankton abundance measurements were used to study macrozooplankton assemblages and migration patterns along Puyuhuapi Fjord and Jacaf Channel in Chilean Patagonia. The dissipation of turbulent kinetic energy was quantified through vertical microstructure profiles collected throughout time in areas with high macrozooplankton concentrations. The acoustic records and in situ macrozooplankton data revealed diel vertical migrations (DVM) of siphonophores, chaetognaths and euphausiids. In particular, a dense biological backscattering layer was observed along Puyuhuapi Fjord between the surface and the top of the hypoxic boundary layer (100 m), which limited the vertical distribution of most macrozooplankton and their DVM, generating a significant reduction of habitat. Aggregations of macrozooplankton and fishes were most abundant around a submarine sill in Jacaf Channel. In this location macrozooplankton were distributed throughout the water column (0 to ∼200 m), with no evidence of a hypoxic boundary due to the intense mixing near the sill. In particular, turbulence measurements taken near the sill indicated high dissipation rates of turbulent kinetic energy (ε10-5 W kg−1) and vertical diapycnal eddy diffusivity (Kρ10-3 m2 s−1). The elevated vertical mixing ensures that the water column is well oxygenated (3–6 mL L−1, 60 %–80 % saturation), creating a suitable environment for macrozooplankton and fish aggregations. Turbulence induced by tidal flow over the sill apparently enhances the interchange of nutrients and oxygen concentrations with the surface layer, creating a productive environment for many marine species, where the prey–predator relationship might be favored.

1 Introduction
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Spatial and temporal variability in plankton assemblages have been linked to oceanographic features and processes such as water column stratification, tidal mixing and turbulence, frontal structures, advection, and secondary circulation in estuaries and fjords (Govoni et al., 1989; Rodriquez et al., 1999; Lee et al., 2005; Lough and Manning, 2001; Munk et al., 2002; Meerhoff et al., 2013, 2015). In fjords, residual flows resemble typical estuarine gravitational circulation with landward flow at depth and seaward flow at the surface. It has been found that residual flows in fjords can retain planktonic larvae inside the system (Dyer, 1997; North and Houde, 2001, 2004; Meerhoff et al., 2015). Another recent study has shown that advection can influence the import and export of zooplankton in a fjord depending on the depth at which the zooplankton are located, which can potentially affect the community composition, biomass, productivity and distribution of zooplankton in the fjord (Basedow et al., 2004). Moreover, horizontal mixing of along-channel density gradients has been shown to induce lateral circulation (Farmer and Feeland, 1983), which in turn affects larval distributions in fjord systems (Meerhoff et al., 2015).

https://www.ocean-sci.net/14/1185/2018/os-14-1185-2018-f01

Figure 1Study area in relation to South America and the Pacific Ocean shown in the inset. The main figure enlarges the study area (Puyuhuapi Fjord and Jacaf Channel) and indicates the instruments used for data collection, fixed point station positions and the sill location near the head of Jacaf Channel. The contours indicate the depth of the fjords.

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Other recent studies have investigated how tidal asymmetries in mixing, and thus tidal variations in stratification, affect ichthyoplankton and zooplankton assemblages (Oviatt, 1981; Lee et al., 2005). Lee et al. (2005) found that variations in stratification throughout a tidal cycle affected both overall abundance and species composition of zooplankton in the Irish Sea. However, they did not have the tools to relate the hydrodynamic and hydrographic variability in this region to vertical and horizontal distributions of fish larvae and zooplankton. Another study by Oviatt (1981) found that zooplankton concentrations were lower in laboratory tanks than in nature (Narragansett Bay, USA). Since this was not due to the physical action of mixing (induced by paddles in the tank), they hypothesized that tank confinement and turbulence had broken down vertical segregation between adults and juveniles, resulting in increased cannibalism. While vertical segregation of zooplankton groups, probably related to different trophic guilds, has been confirmed by several studies (e.g., Haury et al., 1990), this segregation can be reduced by turbulent processes enhancing contact between prey and predators (Visser and Stips, 2002; Visser et al., 2009). For instance, available theoretical models predict optimal prey consumption at dissipation rates of turbulent kinetic energy (ε) between 10−6 and 10−4 W kg−1 (Lewis and Pedley, 2001). In fjords, topographic conditions are extremely irregular (Inall and Gillibrand, 2010), inducing high turbulence and enhanced vertical mixing, particularly at sills (Klymak and Gregg, 2004; Whitney et al., 2014). However, enhanced productivity, oxygenation and/or local retention may occur at these same highly turbulent areas. For example, turbulence is known to mix freshwater inflow with deep, dense ocean water, allowing for oxygenation of the deeper layers (MacCready et al., 2002; Peters and Bokhorst, 2001) and these turbulent eddies can impact phytoplankton bloom growth (Cloern, 1991; Koseff et al., 1993). Therefore, additional field studies are needed to confirm the relationship between mixing-induced physical forcing, such as wind or advection, and vertical abundance patterns and species composition in fjords and other estuarine systems. One of the principal questions that will be addressed in this study is what is the contribution of turbulence to the mixing of fjord water column properties (e.g., temperature, salinity and dissolved oxygen) and to the aggregation of macrozooplankton at certain depths (scattering layers) along north Patagonian fjords and channels, emphasizing the role of sills in some locations (e.g., Jacaf Channel, Fig. 1)?

Dissolved oxygen (DO) is the most important dissolved gas in the ocean as it sustains marine life and ensures ecosystems health. Most of the world's oceans are oxygenated; however, there are some regions of low DO, referred to as hypoxic zones, and if their DO concentrations are equal to or close to 0 mL L−1, they are known as “Dead Zones” (Díaz et al., 2001; Ekau et al., 2010; Hauss et al., 2016). Throughout the world's oceans there exist areas where hypoxic conditions are permanent and where the DO is significantly lower than well-oxygenated areas (e.g., <20µM or 0.31 mL L−1). These areas are known as oxygen minimum zones (OMZs) and due to the upwelling associated with them, they experience elevated primary production at the surface (Mass et al., 2014; Hauss et al., 2016; Seibel et al., 2016). The major ocean OMZs are located in the eastern South Pacific and North Pacific, the Arabian Sea, the Bay of Bengal (Indian Ocean), west Bering Sea, the Gulf of Alaska, and the eastern North Atlantic, covering around 8 % of the total ocean (∼30 million km2; Paulmier and Ruiz-Pino, 2009; Fuenzalida et al., 2009; Hauss et al., 2016). The eastern South Pacific OMZ (ESP-OMZ), present along the Chilean coast, represents an area of 9.8 million km2 (2.6 % of the total ocean; Fuenzalida et al., 2009). Even the ESP-OMZ decreased and disappeared south of ∼37 S; however, water with low DO (2–3 mL L−1), associated with the Equatorial Subsurface Water (ESSW), is still present up to 44 S (Silva et al., 2009). The ESSW infiltrates Patagonian fjords and channels and moves northward and southward (41.5–44 S) depending on the marine topography (Sievers and Silva, 2008).

Hypoxic conditions (<2 mL L−1) have been detected in four regions of Patagonia (Puyuhuapi Fjord, Jacaf Channel, Aysén Fjord and the Almirante Montt Gulf), and in each region the oxygen depleted zones are mainly located at the fjord heads and down to 100 m of depth (Silva and Vargas, 2014; Schneider et al., 2014). Some of the main contributors to hypoxia in Patagonian fjords and channels have been found to be (1) water column stratification causing separation between poorly oxygenated bottom water and oxygenated surface waters, (2) DO consumption by degradation of organic matter (autochthonous and allochthonous), (3) low ventilation due to the presence of deep bathymetric micro-basins, (4) advection of the ESSW and (5) anthropogenic activities such as industrial and sewage discharge, riverine inputs of nutrients, agriculture activities, aquaculture, etc. (Sievers and Silva, 2008; Silva and Vargas, 2014; Schneider et al., 2014).

Hypoxia is known to have a significant impact on plankton distribution and development, hence on the health of the ecosystem as a whole (Ekau et al., 2010; Mass et al., 2014; Hauss et al., 2016; Seibel et al., 2016). Some species can tolerate hypoxic water, e.g., smaller species, euphausiids and jellyfish can live in under 30 % oxygen saturation and dissolved oxygen concentrations of 1.6 mL L−1. Other taxa, such as some copepods and fishes, may be more sensitive to hypoxia and have preference for oxygen saturations of 50 %–100 % and DO concentrations of 2.6–5.2 mL L−1 (Ekau et al., 2010; Mass et al., 2014; Hauss et al., 2016; Seibel et al., 2016). The sensitivity of species to tolerate different oxygen concentrations, however, may vary among organisms from different environments, e.g., coastal upwelling zone, fjords systems and OMZs. Although hypoxic conditions have been detected in four regions of Patagonia (Silva and Vargas, 2014; Schneider et al., 2014), no relationship has been determined with the zooplankton species that inhabit this ecosystem. Therefore, the second question that motivates this study is how do hypoxic conditions affect the distribution and aggregation of macrozooplankton species? This question will be addressed by investigating Puyuhuapi Fjord and Jacaf Channel, two of the four hypoxic ecosystems in Patagonia.

In Patagonian fjords, a comprehensive description of zooplankton distribution patterns has been provided by Palma (2008), considering a total of 220 in situ plankton samples from a number of depth strata between the surface and ∼200 m. Main zooplankton groups included siphonophores, chaetognaths, cladocerans, copepods and euphausiids. Although a positive north-to-south gradient in the abundance of major zooplankton species was found, potential relationships between the vertical distributions and environmental variables were not deeply assessed. A later study by Landaeta et al. (2013) investigated the vertical distribution of microzooplankton and fish larvae in Steffen Fjord (47.4 S) at four depth strata (0–10, 10–25, 25–50 and 50–100 m) during November 2008. Copepod nauplii and copepodites of Acartia tonsa together with Maurolicus parvipinnis fish larvae were observed around the pycnocline region, suggesting that the vertical structure of the water column might play a role in larval fish distribution. More recently, studies on zoo- and ichthyoplankton vertical distributions in the Reloncaví Fjord revealed that DVM timing might be modified by the tidal regime that is particularly strong in this area (Castro et al., 2014). However, none of these studies provided explicit assessments of the relationships between the vertical distribution of zooplankton and turbulent mixing or water column properties.

Most studies carried out in Chilean coastal waters, including those mentioned above, have relied on plankton nets and other collecting devices (pumps) deployed in single locations (fixed stations). An alternate approach is to use acoustic techniques, which can provide high-resolution data on zooplankton DVM patterns (Valle-Levinson et al., 2014; Díaz-Astudillo et al., 2017) and segregation patterns throughout the water column (Sato, 2013; Sato et al., 2016). For instance, DVM patterns of dense krill aggregations have been detected using acoustic Doppler current profilers (ADCP) moored around the Antarctic Peninsula, the Kattegat Channel and off Funka Bay, Japan (Buchholz et al., 1995; Lee et al., 2004; Zhou and Dorland, 2004; Brierley et al., 2006). In Chilean fjords, ADCPs have been used to identify changes in vertical distribution and DVM patterns of zooplankton (e.g., from normal diel to twilight vertical migrations) over several months in the Reloncaví Fjord (Valle-Levinson et al., 2014). These studies, although novel at describing temporal variations in zooplankton patterns, focused mainly on the behavior of a particular species, but again did not consider how the vertical distribution of zooplankton is modified by water column conditions (e.g., temperature, salinity, oxygen and turbulence).

Compared to ADCPs, scientific echo sounders are characterized by narrower beam angles, lower frequencies and longer ranges. They have also been used to provide valuable qualitative and quantitative information on various aquatic species and communities, from zooplankton to large predators (Ballón et al., 2011). Overall, macrozooplankton can be acoustically identified and virtually separated from other organisms, such as fish, by considering their acoustic properties (Logerwell and Wilson, 2004; Mosteiro et al., 2004; Simmonds and MacLennan, 2005). Although the use of several frequencies does not necessarily increase precision (Horne and Jech, 1999), the use of at least two frequencies (38 and 120 kHz) is currently a standard practice in zooplankton studies as identification methods developed by Ballón et al. (2011) and others can be utilized.

The present study aims to evaluate the effects of water column properties, such as dissolved oxygen and turbulent mixing, on the vertical distribution of dominant macrozooplankton groups along a Patagonian fjord system. To achieve this goal, ADCP and scientific echo-sounder data were combined with biological observations from in situ stratified zooplankton samples and water column measurements from microstructure profilers and conductivity–temperature–depth–oxygen (CTDO) profilers. According to the information presented in this section, the principal hypotheses of this manuscript are (1) the pervasive hypoxic layer existing in the Puyuhuapi Fjord limits DVM and the overall distribution of macrozooplankton to the first 100 m depth of the water column, reducing the habitat of these species and (2) the higher turbulence originated by the tidal regime around sills favors the mixing of the water column, deepens the hypoxic layer, injects nutrients and, thus, increases primary production. Therefore, macrozooplankton exhibit higher densities and extend deeper in the water column around submarine sills.

2 Study area
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Patagonian fjords extend from 41 to 56 S, and are typically deep and narrow as a result of their formation during glacial progression. Their hydrography is characterized by two vertical layers, consisting of a low salinity surface layer in the first 10 m of the water column (resulting from rainfall and glacial melt) that overlays a subsurface salty layer originated in the Pacific Ocean (Silva and Calvete, 2002; Pérez-Santos et al., 2014). Fjord systems play an important role in primary production and carbon cycling by providing a zone where energy and particulate material are exchanged between land and marine ecosystems (Gattuso et al., 1998). The principal nutrient (nitrate) is supplied to these fjords by oceanic transport, and particularly through the intrusion of Sub-Antarctic Water (SAAW), a water mass that may also transport some species of zooplankton (González et al., 2011, 2013).

Puyuhuapi Fjord and Jacaf Channel are representative examples of the Patagonian fjord systems. The main connection of Puyuhuapi Fjord with oceanic waters is via its southern mouth. Although a second connection to oceanic water exists via Jacaf Channel, interchange here is limited by the shallow Jacaf Channel sill, which is 50 m deep and 6 km long. Its main freshwater input (the Cisnes River) meets the fjord half way between its head and mouth (Fig. 1). Jacaf Channel is well known for its great depth (>400 m) around its connection to the Moraleda Channel, which contrasts with its very shallow sill near its connection with Puyuhuapi Fjord (Fig. 1). Seasonal hydrographic measurements along Puyuhuapi Fjord have shown a stratified water column except in late winter, when the water column became partially mixed due to a reduction in freshwater supply from rainfall and glacial melting (Schneider et al., 2014). Hypoxic conditions have been detected in Puyuhuapi Fjord below 100 m depth, where oxygen concentrations have been found to be as low as 1–2 mL L−1 (Schneider et al., 2014; Pérez-Santos, 2017). This observed oxygen depletion could be caused by limited ventilation due to shallow sills, or by the input of low-oxygen Equatorial Subsurface Water into the fjord (Silva and Vargas, 2014; Schneider et al., 2014). Puyuhuapi Fjord is the only northwestern Patagonian fjord known to experience such extreme hypoxic conditions. At the same time, it is an area where intense aquaculture activities have been recently developed, which reinforces the need for this study.

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Figure 2(a, c, e ,g) Profiles of temperature (a, b), salinity (c, d), dissolved oxygen (e, f) and nitrate (g) collected during different oceanographic campaigns in the northern central part of Puyuhuapi Fjord and (b, d, f) in the eastern region of the Jacaf Channel.

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Figure 3(a) Volume backscattering strength (Sv) calculated from the ADCP-1 backscatter signal in Puyuhuapi Fjord, deployed at 50 m depth from the 8 to 26 May 2013. (b) Zoom of the Sv data and the times of in situ zooplankton sampling (black dots) carried out during 25–26 May 2013. (c, d) Vertical abundance of main zooplankton groups (>5 mm length) from the in situ sampling at 16:00 and 18:00 (local time) on 25 May and (e, f) at 09:00 and 11:00 (local time) on 26 May.

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Table 1Data set collected during oceanographic campaigns in Puyuhuapi Fjord and Jacaf Channel.

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The study area offers an excellent opportunity for studying the impact of deep hypoxia upon macrozooplankton distribution and behavior, considering the continued increase in hypoxic regions around the world (Breitburg et al., 2018). Moreover, the presence of a sill in Jacaf Channel, in the vicinity of its connection to the Puyuhuapi Fjord, opens the possibility to investigate the influence of vertical mixing (Farmer and Freeland, 1983; Inall and Gillibrand, 2010) upon water quality, especially upon dissolved oxygen concentration, injection of nutrients from subsurface oxygen rich layers, enhancement of primary production and, finally, upon the density of different zooplankton species (Pantoja et al., 2011). Furthermore, the location of an oceanographic buoy in the northern part of Puyuhuapi Fjord (Schneider et al., 2014) is a useful platform to carry out in situ experiments combined with oceanographic moorings.

3 Data collection and methodology
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3.1 Water column properties

Hydrographic surveys were conducted during May and November 2013 and January and August 2014 in Puyuhuapi Fjord and Jacaf Channel (Fig. 2, Table 1). These profiles were obtained with a SeaBird 25 CTDO, sampling at 8 Hz with a descent rate of ∼1 m s−1. The data collected, whose nominal vertical resolution was 12 cm, were averaged into 1 m bins, following SeaBird recommendations. The conservative temperature (C) and absolute salinity (g kg−1) were calculated according to the Thermodynamic Equation of Seawater 2010 (IOC et al., 2010). Additionally, nitrate samples were taken using a Niskin bottle at various depths and analyzed spectrophotometrically following the methods of Strickland and Parsons (1968). To validate CTDO measurements, in situ oxygen samples were analyzed using the Winkler method (Strickland and Parsons, 1968), carried out using a Metrohm burette (Dosimat plus 865) and an automatic visual end-point detection (AULOX Measurement System).

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Figure 4(a) Volume backscattering strength (Sv) calculated from the ADCP-2 backscatter signal in Puyuhuapi Fjord from 22 to 24 January 2014. The in situ zooplankton sampling (in 03:00 intervals) are represented by black dots at the surface. (b) Depth-integrated abundance of zooplankton from the surface to 100 m depth varying throughout time, where (b) is zooplankton >5 mm in length. (c) Vertical abundance of the principal zooplankton groups on 23 January at 02:00 (nighttime) and (d, e, f) same as (c) but on 23 January at 08:00 and 14:00 (daytime) and 24 January at 02:00 (nighttime). The time reference is in local time.

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Microstructure measurements were collected using a vertical microstructure profiler (VMP-250, Rockland Scientific, Inc.). The VMP-250 is equipped with two airfoil shear probes and two fast-response FP07 thermistors, which allowed for data recording at 512 Hz with a descending free fall speed of ∼0.7 m s−1. The micro-shear measurements permitted a direct measurement of the dissipation rate of turbulent kinetic energy (ε) for isotropic turbulence, according to Lueck et al. (2002), Eq. (1),

(1)ε=7.5νuz2,

where ν is the kinematic viscosity, u is the horizontal velocity, z is the vertical coordinate axis and therefore uz2 is the shear variance.

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Figure 5Single-frequency (38 kHz) scientific echo-sounder transect conducted along the Puyuhuapi Fjord during the summertime field campaign (January 2014). Distribution indicated by colors representing Sv. (a) Daytime transect of echo-sounder measurements (Sv) throughout depth (y axis) from the mouth (0 km) to the head (80 km) of Puyuhuapi Fjord on 22 January 2014. (b) Average profiles derived from the nautical area scattering coefficient (NASC) from the daytime transect with standard deviation bars. (c) Same as (a), but for the nighttime starting at 21:57 (local time) 24 January through early in the morning of 25 January 2014. (d) Same as (b) but for the nighttime. The ADCP-2 mooring location is marked with a black dot in (a) and (c). (e) Dissolved oxygen profiles (black dots) obtained approximately every 3 h (close to the position of ADCP-2 mooring) from 23 to 24 January 2014. The location of the hypoxic boundary layer is depicted by the white contour line of 2 mL L−1.

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Figure 6Scatter plot of volume backscattering strength (Sv) from 38 kHz frequency and the most abundant macrozooplankton species obtained in the in situ fixed stations carried out in Puyuhuapi Fjord (a, b, c) during 22–24 January 2014 and in Jacaf Channel (d, e) during 18–19 August 2014.

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Using the values of ε, the diapycnal eddy diffusivity (Kρ) was calculated. The most used formulation was proposed by Osborn (1980),

(2)Kρ=ΓεN2,

where Γ is the mixing efficiency, generally set to 0.2 (Thorpe, 2005), and N is the buoyancy frequency. Shih et al. (2005) noted that when the ratio ενN2 is greater than 100, Eq. (2) results in an overestimation. Therefore, they proposed a new parameterization for this case given by

(3)Kρ=2ν(ενN2)1/2.

More recently, Cuypers et al. (2012) used Eq. (2) when ενN2>100, Eq. (2) when 7<ενN2<100, and considered null eddy diffusivity when ενN2<7. This approach was followed in this study. The correlation between the dissipation rate of turbulent kinetic energy and the abundance of major zooplankton groups throughout the water column was accomplished by using a quadratic polynomial curve fit between these data sets (explained in detail in Sect. 4.6). These analyses were only applied to measurements collected at the fixed station in Puyuhuapi Fjord, because the VMP-250 was not available during the measurement campaign in Jacaf Channel.

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Figure 7Dual-frequency (38 and 120 kHz) scientific echo-sounder transects along Puyuhuapi Fjord (0–18 km) and Jacaf Channel (18–35 km) during nighttime on 17 August 2014. (a) Fluid-like and (b) blue noise echograms for zooplankton and (c) the fish echogram. Distribution indicated by colors representing Sv values. The black arrow in (a) represents the entrance to Jacaf Channel. Horizontal red lines in (a, b, c) denote lower limits of usable acoustic data (250 m).

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3.2 Acoustic data

Three types of acoustic data were collected: ADCP, single-frequency echo-sounder and dual-frequency echo-sounder data. ADCP measurements were obtained with two 307.7 kHz Teledyne RDI Workhorse ADCPs, moored upwards at depths of ∼50 m (ADCP-1) and ∼100 m (ADCP-2), both moored at the same location in north-central Puyuhuapi Fjord but during different time periods (Table 1, Fig. 1). Data were collected hourly with a vertical bin size of 1 m, over periods of austral autumn (ADCP-1: May 2013) and spring–summer (ADCP-2: January 2014). During the final ADCP-2 mooring deployment, single-frequency data were also collected along the Puyuhuapi Fjord using a SIMRAD EK60 scientific echo sounder, running a 38 kHz transducer (ES38B), during daytime and nighttime hours, from 22 to 25 January 2014 (black line in Fig. 1). These ADCP and single-frequency echo-sounder measurements were complemented by in situ zooplankton sampling (see Sect. 3.3 for details) carried out on 23–24 January 2014, at a fixed station close to the ADCP mooring location, over a period of 36 h (Fig. 1).

A second scientific campaign was conducted on 17 and 19 August 2014, which included a dual-frequency echo-sounder survey and a third ADCP mooring (ADCP-3) located in Jacaf Channel. This time, the echo-sounder survey coverage was extended to eastern Jacaf Channel (Fig. 1, red line) and a second 120 kHz transducer (ES120-7C) was added to the 38 kHz transducer used in the first survey. Several day/night transects were completed across Puyuhuapi Fjord and Jacaf Channel, with special attention paid to Jacaf sill (only the most representative echograms area shown in Figs. 5, 7 and 8). To determine the statistical relationship (R2) between acoustic data from the 38 kHz echo sounder with hydrographic properties of the fjords (temperature, salinity and dissolved oxygen), a quadratic polynomial curve was also applied between these data sets. During this survey, two RDI Workhorse ADCP with 614.4 kHz frequency (referenced hereafter as ADCP-3) and was moored at ∼30 m depth in the vicinity of the Jacaf sill. The near-surface placement of ADCP-3 allowed for near-surface currents to be adequately quantified.

Vessel speed during all echo-sounder surveys was maintained between 8 and 10 knots. Echo sounders were operated using a variable ping rate of 0.3–2.0 pings per second, a pulse duration of 1.024 ms and output powers of 2 and 0.5 kW for the 38 and 120 kHz frequencies, respectively. Calibration was made using copper spheres and standard procedures (Foote et al., 1987).

3.2.1 Echo-sounder data analysis

Post-processing of echo-sounder data was performed in Echoview (Myriax Inc, Tasmania, https://www.echoview.com/, last access: 2 October 2018), where noisy data considered as those collected with weak pings, in blind areas, in the near-field, with background noise or subjected to rainbow phenomenon were regarded as “bad data” and were eliminated. After this initial scrutiny and filtering step, all single-frequency echoes (38 kHz, campaign 1) of intensity >−110 dB were considered and treated as a single “biological backscattering” class, which pooled all biological groups present in the study area. Dual-frequency echoes, however, were classified into three different groups following Ballón et al. (2010). These authors created an algorithm, freely distributed as an Echoview template (“FishZpkPeru38&120.evi”), which uses both mean volume backscattering (MVBS) differences (ΔMVBS) and summations (MVBS) between 38 and 120 kHz to discriminate echoes into three different biological backscattering classes: fish and two macrozooplankton groups (macrozooplankton or “fluid-like” and gelatinous or “blue noise” organisms). The fluid-like group follows a sphere model (Holliday and Pieper, 1995) considered appropriate to represent cylindrical and spherical shapes, including euphausiids and large copepods, which are dominant macrozooplankton groups off Peru and Chile (Ayon et al., 2008). The algorithm is considered to be useful for 38 and 120 kHz data from targets whose radius is ≥0.5 mm and has a dB difference of 2–19 dB (Ballón et al., 2010, 2011).

Given physical limitations imposed by near-field and sound-absorption effects related to the echo-sounder frequencies used (38 and 120 kHz), we defined and limited our analyses to an effective sampling range between 5 and 250 m. Absorption is greater for the 120 kHz frequency, which exhibits the shortest range, but has a greater vertical resolution than the 38 kHz echo sounder. The 38 kHz frequency, on the other hand, exhibits a much longer range (>1000 m), but limited resolution regarding small zooplankton scatterers. It has been shown, however, to be efficient for studying macrozooplankton distributions of siphonophores, chaetognaths and euphausiids (Mair et al., 2005; Cade and Benoit-Bird, 2015; Ariza et al., 2016).

Volume backscattering strength (Sv, dB re 1 m−1, where re represents referenced) values from the single-frequency and from each of the three dual-frequency virtual echograms were integrated and rescaled into the customary index “nautical area scattering coefficient” (NASC, in units of m2 n mi2, where nmi2 represents square nautical miles), using a grid of 20 m (depth) by 50 m (distance). Since NASC lies on the linear domain, it can be considered proportional to and suitable for indexing targets abundance (Ballón et al., 2011).

Quadratic polynomial models were fit to assess the statistical relationship (R2) between biological scattering (single-frequency integrated data) and the hydrographic variables measured in each fjord (temperature, salinity and DO).

3.2.2 Acoustic data analysis from ADCPs

ADCP echo intensity was converted to mean volume backscattering strength (Sv, dB re 1 m−1), as done for scientific echo-sounder data, following the conversion formula:

Sv=C+10logTx+273.16R2-LDBW-PDBW(4)+2αR+KcE-Er,

where C is a sonar-configuration scaling factor (−148.2 dB for the Workhorse Sentinel), Tx is the temperature at the transducer (C), LDBW is log10 (transmit-pulse length, L=8.13 m), PDBW is log10 (output power, 15.5 W), α is the absorption coefficient (dB m−1), Kc is a beam-specific sensitivity coefficient (supplied by the manufacturer as 0.45), E is the recorded AGC (automatic gain control), and Er is the minimum AGC recorded (40 dB for ADCP-1 and 41 dB for ADCP-2). The beam-average of the AGC for the four transducers was used to obtain optimal results following the procedure in Brierley et al. (2006). Finally, R is the slant range to the sample bin (m), which uses the vertical depth as a correction (Lee et al., 2004). Therefore, R is expressed as,

(5)R=b+L+d2+(n-1)d+d/4cosζccI,

where b is the blanking distance (3.23 m), L is the transmit pulse length (8.13 m), d is the length of the depth cell (1 m), n is the depth cell number of the particular scattering layer being measured, ζ is the beam angle (20), c is the average sound speed from the transducer to the depth cell (1453 m s−1) and cI is the nominal sound speed used by the instrument (1454 m s−1).

Table 2Harmonic analysis implemented to water level time series in Puyuhuapi Fjord and Jacaf Channel.

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3.3 In situ zooplankton sampling

In situ mesozooplankton samples were collected with a WP2 net (60 cm diameter mouth opening, 300 µm mesh, flowmeter mounted in the net frame) towed vertically from 50 m to the surface in May 2013, and with a Tucker Trawl (1 m2 mouth opening, 300 µm mesh with flowmeter) used to obtain stratified oblique tows in January and August 2014 (Table 1). All samples were preserved in a 5 % formaldehyde solution. Zooplankton abundances were standardized to individuals per m3 of filtered seawater. WP2 vertical tows consisted of five depth intervals from surface to 50 m, every 10 m (0–10, 10–20, 20–30, 30–40, 40–50 m).

Stratified Tucker tows considered four depth strata: 0–10, 10–20, 20–50 and 50–100 m in the Puyuhuapi Fjord. In Jacaf Channel, the stratified sampling included five depth strata: 0–10, 10–20, 20–50, 50–100 and 100–150 m. The hauling speed for both nets was between 2–3 knots. Sampling occurred during a 36 h period every 3 h from 22 to 24 January 2014 (Puyuhuapi Fjord) and every 5–6 h from 18 to 19 August 2016 (Jacaf Channel) (Fig. 1, red dots). At all sites and dates, zooplankton species were identified, sorted into functional groups, measured (length) and classified into size classes using a 5 mm length threshold. To determine the correlation (R2) between the Sv records from the 38 kHz transducer and the major macrozooplankton groups (Siphonophores, Chaetognaths and Euphausiids), a quadratic polynomial curve was also applied between these data sets (further details in Sect. 4.3).

3.4 Tidal harmonic analysis

The tidal constituents were computed using HOBO U20 water level loggers and the pressure sensor from ADCP-3 (Tables 1–2, Fig. 1). A tidal harmonic analysis was applied to the sea level time series according to Pawlowicz et al. (2002), which considers the algorithms of Godin (1972) and Foreman (1977, 1978). We classified tides by the dominant period of the observed tide based on the form factor (F), defined by the ratio between the sum of the amplitudes of the two main diurnal constituents (principal lunar declinational, O1 and luni-solar declinational, K1) and the sum of the amplitudes of the two main semidiurnal constituents (principal lunar, M2, and principal solar, S2), F=(O1+K1)/(M2+S2) (Bearman, 1989; where F<0.25 semidiurnal, 0.25<F<1.5 mixed semidiurnal and F>3.0 diurnal).

4 Results
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4.1 Hydrographic features

Temperature profiles collected in Puyuhuapi Fjord and Jacaf Channel showed a similar structure during the winter and summer campaigns (Fig. 2a, b). The largest temperature gradients were found between the surface and ∼70 m depth, ranging from 8.5 to 17 C. A thin, fresh layer (salinity values varied from 11 to 29 g kg−1) was found in the first ∼10 m of the water column below which salinity varied little (29 to ∼34.2 g kg−1), as a result of the presence of Modified Sub-Antarctic Water (MSAAW, salinity between 31 and 33 g kg−1), the Sub-Antarctic Water (SAAW, salinity between 33 and 33.8 g kg−1) and the Equatorial Subsurface Water (ESSW, salinity >33.8 g kg−1) (Fig. 2c, d). Hypoxic conditions (dissolved oxygen below 2 mL L−1 and ∼30 % saturation) were detected in Puyuhuapi Fjord below 100 m depth, with oxygen concentrations between 1 and 2 mL L−1 (Fig. 2e). Deep water in Jacaf Channel was more ventilated, with dissolved oxygen values above hypoxic conditions throughout the water column (Fig. 2f). The hypoxic layer was located over the depth range of the ESSW and oxygen-rich water (3–6 mL L−1) was observed at depths occupied by MSAAW and SAAW. Below 10 m depth, high nitrate concentrations were measured in Puyuhuapi Fjord, but concentrations in the winter (August, 2014) were higher than in fall (May, 2013) and summer (January, 2014) (Fig. 2g). Along with the in situ hydrographic sampling, in situ zooplankton samples were collected and will now be discussed.

4.2 ADCP acoustic data and in situ zooplankton samples

Volume backscatter (Sv) from ADCP-1 (50 m depth, May 2013) showed large variability, ranging from high (−90 to −75 dB re 1 m−1) to low (−115 to −100 dB re 1 m−1) (Fig. 3a). The highest Sv values (>−90 dB re 1 m−1) were recorded during the night hours ( 18:00 to  07:00 LT, local time; with all remaining times for in situ sampling expressed in local time), while minimum Sv values were observed in the daytime ( 07:00 to  18:00) suggesting that vertically migrating organisms from deeper waters (below ADCP-1 mooring depth of 50 m) migrate upwards during nighttime hours. From the in situ measurements of macrozooplankton collected at various depth strata in May 2013, the most abundant groups were siphonophores, chaetognaths and medusae (Fig. 3c–f). A marked change in vertical distribution and in total abundance of the macrozooplankton groups in the water column was observed from the first sampling hour (Fig. 3c) to the night sampling time (∼18:00 h), revealing the start of the nocturnal migration to the surface (Fig. 3d) coincident with a DVM pattern as seen in the ADCP-1 backscatter data (Fig. 3a, b).

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Figure 8Dual-frequency (38 and 120 kHz) acoustic transect across Jacaf sill conducted during daytime on 18 August 2014. (a) Fluid-like echogram, (b) blue noise echogram for zooplankton and (c) the fish echogram. Distribution indicated by colors representing Sv values. Horizontal red lines in (a, b, c) denote lower limits of usable acoustic data (250 m).

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Data from the ADCP-2 mooring (positioned deeper but at the same location as ADCP-1) from 22 to 24 January 2014 also showed a strong macrozooplankton DVM pattern, which extended down to ∼100 m depth (Fig. 4a). During daylight hours (8:00–18:00), dense aggregations were observed between 80 and 100 m depth, which started to ascend from 18:00 to 21:00, concentrated close to the surface at night, and began to descend at ∼06:00. In situ stratified sampling showed the most abundant macrozooplankton groups were euphausiids, siphonophores, chaetognaths, decapods and medusae (Fig. 4b–f). Euphausiids and siphonophores showed higher abundance close to a surface layer (10–20 m) during night hours (Fig. 4c, f) and at deeper layers during the daytime (Fig. 4d, e). However, euphausiids showed the clearest diel vertical migration with maximum abundance between 10 and 20 m layer during night hours, and at ∼100 m depth during the daytime (Fig. 4c–f). The in situ zooplankton samples were complemented by echo-sounder measurements collected along the fjord systems during the summertime and the wintertime. These measurements will now be discussed.

4.3 Echo-sounder data

4.3.1 Summertime single-frequency survey

The volume backscatter during the summer months overall showed DVM of all macrozooplankton species and a downward migration limit of ∼100 m depth due to the presence of hypoxic conditions below this depth. Summer daytime Sv values along the Puyuhuapi Fjord averaged -89.1±7 dB re 1 m−1 and ranged between −110 and −77.3 dB re 1 m−1, from the mouth to the head of the Puyuhuapi Fjord (Fig. 5a). Most biological backscatter was concentrated in the first 100 m of the water column, matching ADCP-2 results, which showed an increase in backscattering towards 100 m depth (Figs. 4a and 5a). Highest daytime NASC values were found around 80 m (above the hypoxic layer), reaching values of 3–3.5 m2 n mi2 (Fig. 5b). Although some backscatter occurred within the hypoxic layer (below ∼120 m depth), all dense aggregations were observed above it (Fig. 5e).

Summer nighttime biological backscattering along the Puyuhuapi Fjord (Fig. 5c) showed maximum Sv values near the surface, suggesting an ascending vertical migration of all biological backscatter. NASC profiles also showed both an increase in maximum abundances and a shift in the vertical position of the maximum values from 60–80 m during daytime to 40–60 m depth during nighttime (Fig. 5d). Although the water column depth extended to ∼300 m, all dense backscatter aggregations were observed above 100 m depth during both daytime and nighttime hours (Fig. 5a, c). As DO concentrations decreased from 2 to 1 mL L−1 below 100 m depth, biological scatterers in Puyuhuapi Fjord appeared to prefer oxygen concentrations between 3 and 7 mL L−1 (Fig. 5e). The correlation between Sv values and the observed density of different zooplankton groups (in situ samples, >5 mm) was moderate. Such correlations reached values of R2=0.50, for siphonophores (Fig. 6a), R2=0.48 for chaetognaths (Fig. 6b) and R2=0.72 for euphausiids (Fig. 6c). The wintertime sampling showed similar findings but was able to capture more activity in the water column due to the use of two acoustic frequencies.

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Figure 9(a–d) In situ stratified zooplankton sampling along Jacaf Channel during 17 August 2014 and the acoustic data collected simultaneously using the dual-frequency (38 and 120 kHz) echo sounder. FL is fluid-like and BN the is blue noise group. (e) Depth-integrated abundance of macrozooplankton groups from surface to 150 m depth for various sampling hours. (f) Shows the station positions. (g–j) The vertical abundance of the main macrozooplankton groups found during the wintertime survey.

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4.3.2 Wintertime dual-frequency surveys

Wintertime dual-frequency survey data, carried out along Puyuhuapi Fjord and Jacaf Channel on 17 August (∼35 km total transect length, Fig. 1), which allowed for the separation of total backscatter into Fish, Fluid like (FL) and Blue noise (BN) groups (Fig. 7a, b). Total backscatter (Sv) in Puyuhuapi Fjord (0–18 km) showed elevated values in the first 100 m of the water column, but at slightly deeper depths (50–100 m) than in summer (Fig. 5), possibly due to bad weather conditions encountered on the sampling day. Greater intensity (−80 to −60 dB re 1 m−1) and vertical distribution range (0–220 m) of biological backscattering values (Sv>−110 dB) were observed in Jacaf Channel, particularly around its sill (between km 18 and 32; Fig. 7). Particularly high intensities were attributed to BN and FL groups at either side of Jacaf Channel sill on both 17 and 19 August 2014 (Figs. 7 and 8). An important degree of vertical segregation between BN and FL groups was also observed along Jacaf Channel, with the first group concentrated between 100 and 140 m, while the second was between 120 and 200 m (Figs. 7 and 8).

Continuous acoustic sampling repeated over the Jacaf Channel sill confirmed the presence of two backscattering layers: one denser layer between 100 and 150 m and a second less dense layer from 200 to 250 m (Fig. 8a, showed only the best echogram). In situ zooplankton sampling along the Jacaf Channel sill (Fig. 9f) allowed for the detection of the major macrozooplankton (e.g., chaetognaths, euphausiids and crustaceans) found during this experiment (Fig. 9a–d). In general, all sampling stations were carried out during daytime, but station 4 coincided with the ascending moment of macrozooplankton, and highlighted the presence of euphausiids during this time of vertical migration (Fig. 9d). Also, station 1 showed the dominance of crustaceans in the 0–10 m strata. Overall the in situ zooplankton sampling and the echograms showed good agreement with the FL group (Fig. 9a–d). Furthermore, the elevated abundance of macrozooplankton groups (euphausiids and chaetognaths) found between 100–150 m depth during daytime hours (Fig. 9b–f) matched well with acoustic data for the fluid-like group (Fig. 8a), but in the case of the BN group the macrozooplankton species were not clearly identified in the in situ zooplankton sampling.

A moderate correlation was found between Sv values from Jacaf Channel and zooplankton density calculated from in situ samples (>5 mm), with R2=0.42 for Sv vs. chaetognaths (Fig. 6d) and R2=0.41 for Sv vs. euphausiids (Fig. 6e). Now the relationships between water column properties such as temperature, salinity and DO will be compared to the acoustic and in situ macrozooplankton measurements.

4.4 Relationships between biological scattering and water column properties

To examine relationships between the distribution of biological scattering and water column properties, Sv values quantified from the 38 kHz acoustic profiler were matched to the consecutive time at which CTD and DO data were captured. This was done in Puyuhuapi Channel and Jacaf Channel during the summer and winter seasons, respectively. The relationship between water temperature and Sv was weak during summer (R2=0.30) and winter (R2=0.41), with maximum Sv values occurring between 8 and 10 C. A weak relationship was found between Sv and salinity in Puyuhuapi Fjord (R2=0.29) and Jacaf Channel (R2=0.35), with higher Sv values found in the MSAAW and SAAW water masses (salinity >31 g kg−1). Both in Puyuhuapi Fjord and Jacaf Channel, Sv with DO and oxygen saturation showed the highest R2 values (R2∼0.6). Hence, only 20.4 % of total Sv>−110 dB re 1 m−1 were in the hypoxic layer of Puyuhuapi Fjord, while just 1.2 % were in the hypoxic layer in Jacaf Channel. Now the turbulent kinetic energy dissipation will be discussed to relate macrozooplankton assemblages to vertical mixing in the water column.

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Figure 10Profiles of water temperature (blue line), vertical shear (red line) and dissipation rate of turbulent kinetic energy (black line with green dots) obtained with the VMP-250 microprofiler at the depth of the Jacaf sill (∼140 m depth) in (a) Jacaf Channel on 21 November 2013, (c) Puyuhuapi Fjord on 22 November 2013 and (e) in Puyuhuapi Fjord on 23 January 2014. (b, d, f) Representative spectra of velocity shear (u/z) for shear probe 1 (blue line) and shear probe 2 (red line) in wave number space in Jacaf Channel on 21 November 2013, Puyuhuapi Fjord on 22 November 2013 and Puyuhuapi Fjord on 23 January 2014, respectively. The black line denotes the dimensional Nasmyth spectrum and the red and blue triangles the cut-off of maximum wave number (kmax) for each shear probe. The shear spectra were carried out in the same layer (135–145 m) for all turbulence profilers.

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4.5 Tidal regime

The harmonic analysis carried out with the sea level time series obtained in Puyuhuapi Fjord and Jacaf Channel, denoted the dominance (in terms of amplitude) of the semidiurnal constituents (M2 and S2; Table 2). Diurnal constituents (O1 and K1) were also important, specifically at the Jacaf ADCP-3 station located close to the Jacaf sill region (Table 2 and Fig. 1). The contribution of diurnal constituents added the mixed character to the tidal regime in the study area. The spectral analysis implemented at all sea-level stations showed maximum energy in the semidiurnal band (Table 2), with the highest spectral energy (57.29 m2 cph−1, where cph is cycles per hour) at Jacaf sill (Jacaf ADCP-3 station), which could be due to the extreme convergence of the channel at this location accelerating the tidal flows.

4.6 Mixing process

Turbulence measurements collected with the VMP-250 microstructure profiler showed high dissipation rates of turbulent kinetic energy (ε) in the upper 20 m of the water column in Puyuhuapi Fjord and Jacaf Channel (Fig. 10). In this layer, ε ranged from 10−7 to 10−5 W kg−1. However, below this surface layer (<20 m depth) the highest values were obtained around Jacaf sill (ε=1.2×10-7 W kg−1), as shown on 21 November 2013 at 140 m depth (Fig. 10a). In Puyuhuapi Fjord turbulent kinetic energy dissipation between 20 and 180 m was weak (10−10 to 10−7 W kg−1) (Fig. 10c, e). The dissipation rates of turbulent kinetic energy are obtained by integrating the velocity shear spectrum at each respective depth bin up to the noise limit. The noise limit is determined by comparing the measured spectra to the theoretical Naysmyth spectra and determining where the measurements begin to deviate from theory. To display how the estimates of ε were obtained at the Jacaf sill depth, the shear spectra are shown for VMP profiles collected at the Jacaf sill region (21 November 2013 at 140 m depth; Fig. 10b), and in Puyuhuapi Fjord on 22 November 2013 (at 140 m depth; Fig. 10d) and on 23 January 2014 (at 140 m depth; Fig. 10f).

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Figure 11Scatter plots of dissipation rate of turbulent kinetic energy (ε) and (a) volume backscattering strength (Sv) from 38 kHz frequency and (b, c, d) the most abundance macrozooplankton species obtained in the in situ fixed stations carried out in Puyuhuapi Fjord during 22–24 January 2014.

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Figure 12(a) Microstructure profile locations along Jacaf Channel and sill using VMP-250 in November 2013. (b) The color bar showed the dissipation rate of turbulent kinetic energy (ε) and the blue lines depict the velocity shear at each station location along Jacaf Channel (as shown in (a)). The horizontal scale (−2 to 2 s−1) applied to profiles at stations 160, 162 and 163. Station 164 is located at the confluence of Jacaf Channel and Puyuhuapi Fjord (10.5 km). (c) The diapycnal eddy diffusivity profiles (Kp), obtained at each station shown in (a).

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In Puyuhuapi Fjord, the correlation between ε and zooplankton Sv data (38 kHz, fixed station, January 2014) was high (R2=0.65, Fig. 11a). In the same campaign, the in situ macrozooplankton density (>5 mm) was also highly correlated with ε values (R2=0.79 for ε vs. siphonophores, R2=0.66 for ε vs. chaetognaths, and R2=0.77 for ε vs. euphausiids; Fig 11b–d). Unfortunately, VMP data were not collected in Jacaf Channel in wintertime. In order to confirm the relationship between ε and various zooplankton species, additional turbulence measurements were collected in November 2013 along Jacaf sill (Fig. 12a). Results showed strong velocity shear in the horizontal velocities (Fig. 12b) accompanied by high ε values (10−7 to 10−5 W kg−1). Maximum ε was measured at the Jacaf–Puyuhuapi confluence (10 km along transect) at ∼63 m depth where ε=1.9×10-5 W kg−1 (Fig. 12b; St. 164). The diapycnal eddy diffusivity (Kρ) was also high in the same area with values of 10−4 to 10−3 m2 s−1 (Fig. 12c).

5 Discussion
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This study represents one of the first attempts to combine measurements of acoustics, stratified plankton sampling, microstructure profiles and standard hydrographic profiles to investigate both the vertical distribution patterns of macrozooplankton and why these patterns exist in northwest Patagonian fjords and other subantarctic latitudes. Three main findings resulted from this effort. First, DVM patterns of macrozooplankton became evident from all methodological approaches and at all study periods: May 2013, January 2014 and August 2014 (Figs. 3–5 and 7–9). Second, strong evidence arose showing macrozooplankton avoidance of hypoxic layers. And, third, a clear increment of macrozooplankton and fish aggregations around the Jacaf sill could be related to increased turbulence in this area.

5.1 Diel vertical migration patterns

Consistent evidence from multiple echo-sounder surveys, ADCP moorings and semi-continuous in situ zooplankton measurements supported the existence of major circadian displacements of macrozooplankton during night hours between mid-depth (20–120 m) and subsurface waters in our study area. Similar DVM patterns have been found in Reloncaví Fjord (41.5 S), from 300 and 600 kHz ADCP data, by Valle-Levinson et al. (2014) and Días-Astudillo et al. (2017) using a 75 kHz acoustic device. Given a greater resolution, the later work was able to confirm that the DVM affected the whole water column of the fjord (∼200 m). These studies found the presence of euphausiids, decapods, mesopelagic shrimps, copepods and other groups in the Reloncaví Fjord in July and November 2006 (Valle-Levinson et al., 2014), as well as in July 2013 (Días-Astudillo et al., 2017). DVM is a common feature of many zooplankton groups, observed around the world using different ADCP and echo-sounder frequencies, e.g., at the Kattegat Channel (Buchholz et al., 1995), the northeast Atlantic (Heywood, 1996), the northwest coast of Baja California, Mexico (Robinson and Gómez-Gutiérrez, 1998), the northeastern Gulf of Mexico (Ressler, 2002), the Antarctic Peninsula (Zhou and Dorland, 2004), the Arabian Sea (Fielding et al., 2004), Funka Bay, Japan (Lee et al., 2004), south Georgia (USA), in the Atlantic sector of the Southern Ocean (Brierley et al., 2006) and Saanish Inlet, British Columbia, Canada (Sato et al., 2013). The scattering layers observed in these studies highlight the abundances of the major zooplankton species, represented by amphipods, euphausiids, siphonophores, chaetognaths, pteropods, crustaceans, small fish and gelatinous plankton. While most DVM patterns reported in these studies occurred between 0 and ∼300 m depth, the deepest DVM patters were observed in the North Atlantic Ocean, reaching depths ∼1600 m (Van Haren and Compton, 2013).

DVM patterns of zooplankton are expected to be associated with diel changes in visible light within the photic zone (from surface to ∼100 m). Thus, the zooplankton can avoid predators during daytime hours and have safe-feeding conditions at night. While only small irradiance levels, <10−7 times surface levels, can be detected beyond 600 m (Van Haren and Compton, 2013; Sato et al., 2013, 2016), zooplankton DVM can reach depths below 500 m (Van Haren and Compton, 2013). Moreover, zooplankton DVM occurs in Arctic fjords (e.g., the Kongsfjorden and Rijpfjorden fjords) even during the polar night, suggesting a high sensitivity to very low levels of solar and/or lunar light (Berge et al., 2009). Since both Puyuhuapi Fjord and Jacaf Channel are not deeper than 300 m, enough light should reach the bottom layer and stimulate zooplankton DVM across the whole water column. However, our results show that zooplankton DVM (and distribution as discussed in the next section) was limited by the hypoxic boundary layer present in the Puyuhuapi Channel (∼100 m; Fig. 5), providing indirect support to the idea that hypoxia may limit DVM in poorly ventilated Patagonian fjords and elsewhere (Ekau et al., 2010; Mass et al., 2014; Hauss et al., 2016; Seibel et al., 2016).

5.2 Macrozooplankton avoidance of hypoxic waters

In Puyuhuapi Fjord, hypoxic conditions have been reported below ∼100 m depth, all year round (Schneider et al., 2014; Silva and Vargas, 2014), with sporadic deep ventilation events that increase the DO concentration from 1.4 to 2.8 mL L−1 (Pérez-Santos, 2017). These pervasive hypoxic conditions are not common in all Patagonian fjords. For instance, seasonal hydrographic data from Reloncaví Fjord showed well-ventilated conditions along the fjord, with deep, near-bottom DO values between 3 and 3.5 mL L−1 (Castillo et al., 2016).

In the current study, acoustic measurements revealed that most biological backscattering (Sv data) occurred above the hypoxic boundary layer (Fig. 5), which acted as a barrier to DVM and macrozooplankton distribution throughout the year. Similar findings were reported in Oslofjord, Norway, where hypoxic conditions dominated the water column beneath ∼60 m depth, and no fish or krill were observed below this depth (Røstad and Kaartvedt, 2013). Moreover, in the eastern South Pacific OMZ, it has been previously reported that a number of copepod species and life-stages avoid hypoxic waters (Castro et al., 1993; Escribano et al., 2009), as well as for most gelatinous zooplankton groups (Pages et al., 2001; Giesecke and Gonzalez, 2005; Escribano et al., 2009). In the same OMZ region, but further north in Peruvian waters, two diurnal scattering layers were observed, one over the OMZ and another, mainly composed of adult euphausiids, in the core of the OMZ (Ballón et al., 2011). Euphausiids, salps and myctophid fish were also observed in the core of the eastern tropical North Pacific OMZ (Mass et al., 2014). Seibel et al. (2016) reported Euphausia eximia and Nematoscelis gracilis tolerance to hypoxic water and suggest this tolerance would enable these species to reduce their energy expenditure by at least 50 % during their daytime migration.

The highest Sv values observed in Puyuhuapi Fjord occurred at DO concentrations between 2 and 5 mL L−1, while in Jacaf Channel between 3 to 6 mL L−1. DO values of 3.5 and 4.5 mL L−1 seemed to represent appropriate conditions for most macrozooplankton species in Puyuhuapi Fjord and Jacaf Channel, respectively, which are similar to the values indicated by Ekau et al. (2010) for zooplankton. Our results also showed that macrozooplankton preferred oceanic waters with salinity values >31 g kg−1, and temperatures between 8 and 10 C (Figs. 4 and 9). Nonetheless, it must be considered that these preference values were estimated from observational data and limited sampling rather than from controlled experiments.

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Figure 13Conceptual model to show the oceanographic processes that contribute to the distribution and aggregation of zooplankton in (a) Puyuhuapi Fjord and (b) Jacaf Channel.

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Vertical overlapping observed between fish and macrozooplankton abundances suggests that prey–predator interactions might be enhanced under hypoxic conditions. Pollution and climate change are continually expanding the extent of hypoxic waters around the world, both in coastal waters and open oceans (Breitburg et al., 2018). While the links between recent anthropogenic perturbations, such as the salmon aquaculture expansion and hypoxia in Patagonian fjords, are still under debate, it is important to keep this potential impact upon habitat reductions and enhanced prey–predator interactions under consideration as it might cause changes in zooplankton groups' distributions and abundance, particularly those that do not tolerate low DO concentrations.

The fact that some biological backscattering occurred within the hypoxic layer in our study indicates that hypoxia does not affect all macrozooplankton species equally and that some of them can inhabit this deeper layer, e.g., euphausiid species (Mass et al., 2014; Seibel et al., 2016). Hypoxia-tolerant species residing below and within minimum DO layers have been reported, for example, further north along the Chilean coast during the upwelling season, leading support to the hypotheses on predation evasion and horizontal transport aiming to explain such behavior (Castro et al., 2007). Within this context, Euphausia pacifica has been reported to exhibit the highest abundance of zooplankton species present in hypoxic waters in Hood Canal, USA (Sato et al., 2016). Other euphausiids have also been reported to be present in other hypoxic systems in Chile (Escribano et al., 2009; Gonzalez et al., 2016). It has been shown that Euphasia vallentini is a dominant euphausiid species known to carry out extensive vertical migrations in Patagonian fjords, hence we speculate it might be one of the species occurring in the less oxygenated waters of our study. Unfortunately, due to sampling gear restrictions, we were unable to sample the hypoxic layer, nor to firmly identify the species occurring at this depth. Therefore, future research will be necessary to understand the relationship of the deep, yet scarce, macrozooplankton within the hypoxic waters in Puyuhuapi Fjord. As vertical mixing is a mechanism that could reduce the presence of hypoxic zones in fjords, values of turbulent kinetic energy dissipation were compared to the depth strata of macrozooplankton.

5.3 Turbulent mixing at the fjord sill

Patagonian fjords and channels cover an area of ∼240 000 km2 and feature a complex marine topography, including submarine sills and channel constrictions (Iriarte et al., 2014; Inall and Gillibrand, 2010). Bernoulli aspiration, internal hydraulic jumps and intense tidal mixing are all processes that can be found near a fjord sill (Farmer and Freeland, 1983; Klymark and Gregg, 2003; Inall and Gillibrand, 2010; Whitney et al., 2014). Our data showed elevated values of turbulent kinetic energy dissipation in Jacaf Channel (ε=10-5 W kg−1 and Kρ=10-3 m2 s−1) near the sill from 0 to 60 m depth. These values are similar to those observed at the sill of Knight Inlet in Canada (Klymark and Gregg, 2003). Lower ε values were found in Puyuhuapi Fjord (Fig. 10). The elevated vertical mixing (high Kρ) in Jacaf Channel is probably due to the barotropic tide interacting with the submarine sill (Schneider et al., 2014; Figs. 10, 12 and Table 2). This was also observed in Martinez Channel (Pérez-Santos et al., 2014), central Patagonia, where semidiurnal internal tides were found to dominate the estuarine dynamics (Ross et al., 2014). This region is highly influenced by the Baker River, whose discharge enhances stratification and introduces suspended solids that subsequently limit productivity in the water column (González et al., 2010, 2013; Daneri et al., 2012).

The evident aggregation of macrozooplankton and fish found near Jacaf sill (within ∼1 km) matches the area exhibiting the highest ε values (10-5 W kg−1; Fig. 12). Thin (2–5 m) and thick (10–50 m) regions of enhanced vertical shear measured directly with the VMP-250 microstructure profiler contribute to vertical mixing. Subsequently this enhances the exchange between the subsurface, rich nutrient layer (Fig. 2) and the photic layer, leading to increased phytoplankton productivity (Montero et al., 2017a, b), as shown in the conceptual model of Fig. 13. Thus, the acoustic and turbulence measurements collected near Jacaf sill promote the importance of a sill in influencing the vertical distribution of oxygen, macrozooplankton and fish on both sides of the sill.

A summary of the processes that can contribute to macrozooplankton vertical distribution and aggregation in Puyuhuapi Fjord and Jacaf Channel are presented in a Fig. 13. In Puyuhuapi Fjord, at 100 m depth a high nutrient and high production layer (Daneri et al., 2012; Montero et al., 2017a, b) is separated from a hypoxic layer below, which limits species distribution and lacks significant aggregations of zooplankton. Above the hypoxic waters, turbulent mixing enhances contact between macrozooplankton predators and their prey (Visser et al., 2009). In Jacaf Channel, the hypoxic layer occurs deeper in the water column than in Puyuhuapi Fjord, which stretches the vertical distribution of macrozooplankton to a deeper range. Turbulent mixing also increases primary and secondary production, through enhanced nutrient availability and favors encounters of macrozooplankton with potential prey, increasing growth and survival rates (Visser and Stips, 2002; MacCready et al., 2002; Klymak and Gregg, 2004; Lee et al., 2005; Visser et al., 2009; Whitney et al., 2014).

5.4 Other findings and considerations

Results showed similar groups of macrozooplankton (>5 mm) in Puyuhuapi Fjord and Jacaf Channel: euphausiids, chaetognaths, medusae and siphonophores during summer (January 2014) and winter (winter 2014). However, euphausiids were not observed in fall 2013, which was an unexpected result which deserves further confirmation and analysis. In contrast, fall 2013 sampling presented the highest acoustic abundances within the time series (Fig. 3). The elevated accumulation of macrozooplankton species around the sill may impose a significant modification in the amount and quality of carbon exported to deeper waters in particular zones of the fjords. Future studies on carbon flux quantification in fjords should incorporate sill regions to test this hypothesis, in order to improve ocean pumping assessments in the context of climate change and variability.

6 Conclusions
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This paper was aimed to determine how hypoxic conditions affect the vertical distribution of macrozooplankton in fjords and to assess how vertical mixing relates to abundances of macrozooplankton at fjord sills. Results showed that the hypoxic layer in Patagonian fjords limits DVM and overall distribution of macrozooplankton to the upper ∼100 m of the water column, reducing the habitat of these species. The hypoxic zones were found away from underwater sills or areas that would experience enhanced turbulence. When assessing the abundance of macrozooplankton in conjunction with turbulent kinetic energy dissipation near a submarine sill it was found that elevated turbulence generated by the barotropic tide interacting with the sharp bathymetric feature enhanced vertical mixing, deepened the hypoxic layer and injected nutrients. In addition, macrozooplankton were found in higher densities and extended deeper in the water column around the submarine sills. This is thought to be due to an increase in primary production that would result from the effects of elevated vertical mixing.

Data availability
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Data availability. 

All data sets used in this manuscript can be request from the corresponding author.

Author contributions
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Author contributions. 

IPS: study design, collection and analysis of physical oceanographic and acoustic data, and manuscript leader. LR and NM: collection and analysis of physical oceanographic and acoustic data, and manuscript revision. LC and EE: study design, collection and analysis of zooplankton data, and manuscript revision. MG, EN, LC and NA: analysis of acoustic data and manuscript revision. MC: analysis of tidal data. GD: study design, collection of nutrient data and manuscript revision. All authors contributed to the writing of the manuscript.

Competing interests
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Competing interests. 

The authors declare that they have no conflict of interest.

Acknowledgements
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Acknowledgements. 

The ADCP data were collected as part of the FONDECYT grant 3120038 and 11140161 by Ivan Perez-Santos and the help of Wolfgang Schneider's research group. Financial support was also provided by Centro Copas Sur Austral AFB170006. We thank Arnoldo Valle-Levinson for motivating the acoustic study of zooplankton in Chilean Patagonia. We also thank Luis Cubillos and Billy Ernst for providing the scientific echo sounder and Cristian Parra and Hernán Rebolledo for administering the scientific echo-sounder sampling. Leonardo Castro was also financed by Centro de Investigaciones de Altas Latitudes (IDEAL). We thank Juan Ramón Velasquez and Oscar Pizarro's research group for their assistance in the ADCP 1, 2 and 3 moorings and Adolfo Mesa, Aldo Balba and Eduardo Escalona for conducting most of the zooplankton sampling. Giovanni Daneri is funded by FONDECYT grant 1131063. Manuel Castillo is funded by FONDECYT grant 11160500.

Edited by: Markus Meier
Reviewed by: five anonymous referees

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Fjord systems play an important role in primary production and carbon export. Acoustic, hydrographic and in situ abundance measurements were used to study macrozooplankton assemblages at 44.7° S. Diel vertical migration of zooplankton stopped at the hypoxic boundary layer and apparently did not tolerate the hypoxic conditions. Turbulence appears to be the oceanographic process that contributes to vertical mixing around the sill, helping the interchange of nutrients, feeding and carbon export.
Fjord systems play an important role in primary production and carbon export. Acoustic,...
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